More than 30 MPs have signed a motion of no confidence in Commons Speaker Sir Lindsay Hoyle following his handling of a vote on Gaza.
Thirty three people from the Conservative and SNP party backed the Early Day Motion (EDM) following chaos in the chamber.
William Wragg, Sir James Duddridge and Brendan Clarke-Smith are among the Tories who support the call.
The SNP’s David Linden, John McNally and Joanna Cherry also among the signatories.
Sir Lindsay should resign as Commons Speaker, SNP MP Pete Wishart said.
The MP for Perth and North Perthshire, who signed the EDM, said: “I think it’s intolerable, and it’s really difficult to know how you could remain in the chair after what happened today.
“Now I know he came down and took responsibility and said sorry, but quite frankly it’s not good enough.”
He added: “I think (it) requires him to look at his situation as Speaker of the House of Commons.
“And I know that nearly all of my colleagues have signed the early day motion asking for him to go, and speaking to several Conservative colleagues this evening, I know that a great number of them have also signed that too.”
Asked if Sir Lindsay should resign, Mr Wishart said: “Absolutely. I cannot see a route for him to be able to command the respect of the whole of the House which will enable (him) to be in that chair.”
One Tory signatory said: “The speaker has broken the trust of his position and made his position untenable.”
Mr Wishart, who was elected as an MP in 2001, said he has not seen anything like the scenes in the House of Commons on Wednesday despite having served as an SNP MP for more than 20 years.
In 2009, 22 MPs backed an identical motion in Michael Martin.
He resigned two days later.
Sir Lindsay, who was first selected as a LAbour MP but then relinquished his party affiliation of the Speaker role, said: “I thought I was doing the right thing and the best thing, and I regret it, and I apologise for how it’s ended up.”
He said he took the decision to allow all sides to “express their views” and that he was “very, very concerned about the security of all members”.
It had been expected that Sir Lindsay would select just the Government’s amendment seeking an “immediate humanitarian pause” to the Israel-Hamas conflict, which could pave the way for a more permanent stop in fighting.
But instead, he decided that the Commons would first vote on Labour’s calls for an “immediate humanitarian ceasefire” before moving on to further votes on the SNP’s original motion, and then the Government’s proposals if either of the first two were to fail to garner enough support.
The move sparked fury from the Conservative and SNP benches.
